Definition
- Ready-diluted to save gradient preparation time.
- Algrade 40,80 in 25mL , are prepared to minimize sudden pH and osmolarity changes during the transition of sperm from the semen sample to the fertilization medium.
- This can help to avoid premature hyperactivation and improves fertilization potential.
Features
- Can keep pH between 7.4 – 7.8 at room temperature , at 37°C providing suitable conditions for good sperm motility, survival time, and fertilization potential.
- The preparation contains glucose as a usable energy substrate for normal cell metabolism and sperm function.
- The gradient enables motile sperm to be separated from extraneous cells and seminal plasma.
- by the effective removal of immature sperm and lymphocytes, damage to sperm DNA and structural molecules caused by ROS is minimized.
Components
- Silane-coated silica
- Potassium chloride
- Sodium chloride
- Purified water
- HEPES
- EDTA
- Glucose
- Sodium pyruvate
- Calcium chloride
- HSA
Storage and Stability
- Store unopened bottles at 2 to 27ºC and avoid temperatures above or below these values. The expiry date is shown on both bottles and cartons.
- Open and close bottles under aseptic conditions. After opening, store at 2 to 8ºC when not in use.
